Automated TruSeq® Stranded Total RNA Library Preparation Method for Eppendorf epMotion
Hamburg, February, 2014
Today Eppendorf announces the release of the epMotion automation method for Illumina’s TruSeq Stranded Total RNA library preparation kit for next-generation sequencing as “Illumina Qualified.” “Illumina Qualified” indicates that Illumina’s analysis of libraries prepared with this epMotion method has shown the libraries to perform comparably to those prepared manually.
Sample preparation for next-generation sequencing (NGS) involves extensive manual processing that can affect reproducibility. Eppendorf epMotion Liquid Handling systems are important tools for labs that want to significantly increase throughput and consistency of results. The automation of labor-intensive steps in NGS sample prep, like pipetting, mixing, temperature control and magnetic separation, brings about a standardized and streamlined process, with significantly reduced hands-on time and minimization of human errors.
“Eppendorf’s method for TruSeq Stranded Total RNA provides our customers with another great option for high throughput automation,” said Peter Fromen, Senior Director of Product Marketing at Illumina. “Eppendorf is one of the leading providers of automation to NGS users, and we are looking forward to additional methods from them in the near future.”
“The Stranded Total RNA sequencing library preparation using the epMotion minimizes our hands-on time while achieving overall high reproducibility. This streamlines and standardizes our sequencing workflow, thereby allowing for higher sequencing throughput. We are looking forward to collaborating with Eppendorf on additional automated library prep protocols,” said Dr. Wei Chen, Head of the Scientific Genomics Platform at Max Delbrück Centre for Molecular Medicine in Berlin, Germany.
“This method is tailored for 8, 16 or 24 samples of 100 to 1000 ng total RNA and gives sequencing ready libraries in 9 to 12 hours. While this is not much faster than the manual process, the hands-on time is significantly reduced as the automated process needs less than one hour preparation time in total,” said Dr. Reza Hashemi, Executive Director of Portfolio Management Liquid Handling at Eppendorf.
Additional automated methods for NGS library preparation from Illumina are currently in development.
Eppendorf & Science Prize for Neurobiology 2014 - Call for entries
Hamburg, February 2014
Eppendorf and the journal Science are now accepting applications for the 2014 Eppendorf & Science Prize for Neurobiology - an annual international research prize of US$25,000. The prize is awarded to one young scientist for his or her outstanding contributions to neurobiology research based on methods of molecular and cell biology. Researchers who are 35 years of age or younger are invited to apply by June 15, 2014. The prize winner is selected by a committee of independent experts in the field, chaired by Science’s Senior Editor, Dr. Peter Stern.
The 2013 prize was won by the Israeli scientist, Dr. Michael Yartsev, CV Starr Postdoctoral Research Fellow of the Princeton Neuroscience Institute at Princeton University. Dr. Yartsev uses an unusual animal model, the bat, to study the underlying neural mechanisms of spatial memory and navigation in the mammalian brain. His approach allows for both a comparative examination of current hypotheses as well as novel insight into long standing questions in the field. His work also underscores the potential benefits of using new animal models in neuroscience.
For more about the prize and film interview with Dr. Yartsev, go to www.eppendorf.com/prize.
Protect your pipette after over pipetting with new Eppendorf ep Dualfilter T.I.P.S® SealMax filter tips
Hamburg, January 2014
New ep Dualfilter T.I.P.S® SealMax filter tips from Eppendorf are designed to protect samples and pipettes against contamination through aqueous sample liquid during accidental over pipetting, as well as against contamination through aerosols and biomolecules.
In laboratories where research with precious, potentially harmful, samples is conducted, filter tips are mostly used. Working with adjustable volume pipettes can put the user at risk when combining pipettes with filter tip sizes that are beyond their capacity. An over pipetting situation can cause pipette contamination due to the sample passing through the filter. With the new ep Dualfilter T.I.P.S. SealMax, researchers can be confident their pipette is safe.
These filter tips do not only build a reliable barrier against liquid, should accidental over pipetting occur during routine laboratory work. The violet and white filter layers also retain practically 100% of all aerosols and biomolecules. Unlike conventional self-sealing tips, ep Dualfilter T.I.P.S® SealMax filter tips combine two-phase filter protection with an innovative fluid and moisture barrier without the risk of the sample liquid being inhibited (e.g. PCR) after any contact with the filter. When in use, the tips initially seal as the aqueous liquid passes through the first hydrophobic filter layer, built to retain drops and splashes.
The sample then passes into a second filter layer, which creates a reliable barrier against the liquid and protects the pipette against contamination. Further information on specific sample recovery features, PCR inhibition and aerosol protection are available on your local Eppendorf website.
The PCR clean / Sterile (sterile and pyrogen-free) purity grade of Eppendorf’s ep Dualfilter T.I.P.S® SealMax filter tips makes them safe for use across a range of applications. Lot-specific purity certificates are available online.
For more information, visit www.eppendorf.com/consumables.
Count on it - Eppendorf’s new Multipette M4 dispenses up to 100 times without refill and counts all executed dispensing steps
Hamburg, January 2014
Eppendorf’s new Multipette M4 is the latest addition to the company’s hand-held serial pipetting range, designed to minimise the time and effort required for precise and highly accurate repeat dispensing tasks.
The new Multipette M4 works with nine sizes of Eppendorf Combitips advanced. A built-in sensor detects the Combitips automatically, showing the tip volume on an easy to read display and eliminating the need for manual volume calculations. In addition, the new central Combitip ejector supports one-handed operation for contact-free tip ejection that avoids any risk of contamination.
Since there are 20 volume settings for each of the nine Combitips, the Multipette M4 can dispense volumes from 1 µL to 10 mL and completes as many as 100 operations without refill. This is significantly more than similar hand held dispensers on the market. An integral step counter displays the number of steps executed, allowing the user to resume with confidence should there be any interruption while dispensing.
With the ability to conduct a maximum of 100 dispensing actions accurately and precisely, the new Multipette M4 is ideal for applications involving 96-well plates, for example. Based on the positive displacement principle it also eases the handling of viscous or volatile liquids and lowers the risk of contaminating the Multipette itself when dealing with radioactive, toxic or other hazardous materials. Unlike air cushioned pipettes, the Multipette M4 allows accurate and precise dispensing of difficult to handle viscous solutions as well as liquids with high vapour pressure.
Repetitive dispensing procedures, such as those required in plate filling, aliquoting and kit usage, can be very time consuming, with ample opportunity for errors and inconsistencies. The new Multipette M4 provides a new, highly efficient solution for quick and safe dispensing that laboratories can rely on.
More information on Eppendorf’s range of liquid handling instruments can be found at www.eppendorf.com/M4.
Eppendorf launches the new TransferMan® 4r micromanipulator with unprecedented movement control
Hamburg, December 2013
Eppendorf’s new TransferMan 4r is an innovative micromanipulator that combines an intuitive user interface with outstanding precision for rapid and simple microinjection. The exceptionally direct transmission of movement in all directions gives a “real time feeling” and makes this latest instrument in Eppendorf’s micromanipulator line the ideal platform for a broad range of applications, including microinjection and manipulation of suspension cells such as oocytes and blastocysts. The new DualSpeed™ joystick extends the precise and intuitive direct movement control with an additional dynamic speed mode for covering longer distances or speeding up sample processing.
The multifunctional TransferMan 4r simplifies individual workflows by offering four predefined application-specific user interfaces such as “cell transfer” and “DNA injection”. Users also have the option of programming additional methods for their individual application needs.
The ergonomically designed control board and unique DualSpeed joystick mean the TransferMan 4r is tailored to providing excellent precision during cell handling and manipulation. It is extremely resistant to vibration and drift during use, delivering exceptional direct movement control for risk- and stress-less working. In addition, the instrument has a programmable Z-axis limit to prevent accidental capillary breakage. Capillary and angle settings are operated single handedly for increased convenience.
Thanks to the flexible assembly and mounting concept, installation of the TransferMan 4r on all common microscopes is possible. Semi-automatic injections are enabled by combination with the electronic Eppendorf microinjectors. The newly introduced electronic coupling with the Eppendorf PiezoXpert® enables semi-automatic piezo-supported cell penetration.
For more information on the Eppendorf TransferMan 4r, please visit www.eppendorf.com/cellmanipulation.
Research on Spatial Memory in Bats Wins 2013 Eppendorf & Science Prize
The Israeli scientist, Michael Yartsev Ph.D., CV Starr Postdoctoral Research Fellow of the Princeton Neuroscience Institute at Princeton University has won the 2013 Eppendorf & Science Prize for Neurobiology. Dr. Yartsev uses an unusual animal model, the bat, to study the underlying neural mechanisms of spatial memory and navigation in the mammalian brain. The use of the bat, along with the development of techniques to record the activity of single cells in brains of these flying mammals, allowed him to conduct a comparative examination of current hypotheses as well as to provide novel insights into the neural codes underlying the representation of three-dimensional space in the brain. His work also underscores the potential benefits of using new animal models in neuroscience.
Dr. Yartsev writes, “All animals on our planet, either on the ground, the ocean depths, or in the sky, must have knowledge of their whereabouts to survive. How the brain solves the problem of knowing where we are in space is a core question in neuroscience. During my doctoral research in the laboratory of Dr. Nachum Ulanovsky at the Weizmann Institute in Israel, I studied the neural activity of spatially selective neurons in the hippocampal formation of freely behaving and flying bats to gain insight into this problem. Using the bat’s unique behavioral repertoire and sensory systems, I now also plan to study the computations taking place in the brain during decision making processes.”
According to Prize Jury Chair and Science Senior Editor, Dr. Peter Stern, “The results obtained by Michael Yartsev’s work are novel and significant in our understanding of how three-dimensional space is represented in the mammalian hippocampus. They suggest that place cells cover volumetric space uniformly and isotropically. His results point towards the underlying mechanisms of how the brain achieves these astonishing feats.”
The annual US$25,000 Eppendorf & Science Prize for Neurobiology honors scientists, like Dr. Yartsev, for their outstanding contributions to neurobiology research. Dr. Yartsev is the twelfth recipient of this international award. He will be presented with the Prize at a ceremony held during the week of the 2013 Annual Meeting of the Society for Neuroscience in San Diego.
The next deadline for applications for the Eppendorf & Science Prize for Neurobiology is June 15, 2014. For more information about Dr. Yartsev and the Prize, visit www.eppendorf.com/prize.
BioBLU® family grows: New 1 L single-use bioreactor for cell culture and microbial applications
Juelich/Hannover, October 14th, 2013 – At this year’s BIOTECHNICA in Hannover, Germany Eppendorf has presented the newest members of its single-use bioreactor family BioBLU for the first time. With the new single-use bioreactor BioBLU 1c for cell culture and BioBLU 1f for microbial applications Eppendorf further extends its portfolio of rigid wall single-use bioreactors. These 1 L vessels are bridging the gap between the BioBLU 0.3 c/f mini single-use bioreactors and the larger vessels of the BioBLU family. Users in cell culture, who want to combine the advantages of single-use technology with the proven qualities of stirred-tank bioreactor design can now benefit from an unmatched portfolio of rigid wall stirred tank single-use bioreactors with working volumes from 100 mL – 40 L.
The BioBLU 1c/f single-use bioreactors have been specifically developed for the use with DASGIP® Parallel Bioreactor Systems. They are perfectly suited to manage the various challenges in bioprocess development. With DASGIP systems 4, 8 and more bioreactors can be operated in parallel. Both, in cultivation of animal and human cells as well as for microbial applications with bacteria and yeasts users profit by parallel processing. Precise process control for reliable and comparable results, time savings due to parallelization of workflows and minimal setup times of single-use bioreactors strongly accelerate bioprocess development. Effective process planning and automation, Design of Experiment (DoE) and comprehensive bioprocess information management give an extra plus for time and cost effective bioprocessing.
The fully instrumented rigid wall BioBLU 1c/f single-use bioreactors can be operated with working volumes of 320 mL – 1.25 L in cell culture and 250 mL – 1.25 L in microbiology. All critical process parameters such as temperature, pH and dissolved oxygen can be measured and controlled via industry standard sensors. Integrated dip-tubes allow for controlled addition of liquids, sampling and controlled submerged and/or headspace gassing. The BioBLU 1c comes with two 3x45° pitched blade impellers, the BioBLU 1f with three 6-blade Rushton-type impellers. The specifically developed encapsulated, magnet-coupled stirrer supports sterile and powerful agitation with up to 600 rpm in cell culture and 1600 rpm for microbial applications. Mass transfer rates that meet the high demands of microbial applications are guaranteed.
The innovative liquid-free Peltier condenser provides optimal cool down of exhaust. The BioBLU 1f additionally carries four cooling-baffles which support a precise temperature control even with modern high cell density processes that generate extraordinarily high levels of heat.
All wetted materials of the BioBLU 1c/f single-use bioreactors are USP Class VI certified and conform to United States Food and Drug Administration requirements.
New Eppendorf Reference® 2 pipette provides optimum accuracy and reliability for valuable testing work
Hamburg, March 2013
Eppendorf has introduced the new Reference® 2 pipette to its range of liquid handling instruments. The Reference® 2 is the successor to the highly successful Reference® pipette, and enhances the range’s already high standards with a new design, reduced weight and operating forces and multi-channel versions. As the premium product in Eppendorf’s pipette portfolio, the Reference® 2 provides the most accurate results possible alongside robust, reliable handling and optimum user safety. These levels of security make the Reference® 2 ideal for use with precious liquids, or in any application requiring extremely high levels of accuracy.
The Reference® 2 ensures complete traceability through the use of an embedded RFID chip containing all the instrument’s relevant data, including serial number and certificate of conformity. Furthermore, a serial number printed on multiple components of the pipette prevents parts from being mixed up and indicates if one of the volume defining parts has been exchanged. Vital reproducibility is guaranteed by the channel indicator incorporated into the multi-channel version of the Reference® 2. This promises a continuously identical pipette alignment during the work process. The pipette also provides highly flexible use, with the ability to remove single cones, and easy maintenance.
The combination of one-button technology and spring loaded tip cone makes the Eppendorf Reference® 2 exceptionally easy and quick to use. The pipette achieves quick tip ejection with active aerosol reduction, and reduces the possibility of repetitive strain injury by eliminating the need to move your thumb sideways.
When there can be no compromise on quality and precision, Eppendorf’s new Reference® 2 is an ideal instrument for these liquid handling applications. For more information, visit www.eppendorf.com/reference.
Eppendorf & Science Prize Winner 2012 in Hamburg
Hamburg, August 2013
The American scientist, Marlene R. Cohen, Assistant Professor in the Department of Neuroscience at the University of Pittsburgh and 2012 winner of the Eppendorf & Science Prize for Neurobiology, visited Eppendorf headquarters in Hamburg in August 2013.
On this occasion Dr. Cohen gave a talk about her ground-breaking work. She uses the responses of groups of visual neurons to estimate which part of the visual world an animal is paying attention to at any given moment. Her approach provides a new way to study how our mental activity affects what we perceive and how cognitive processes such as attention are controlled.
The international US $ 25,000 Eppendorf & Science Prize for Neurobiology is awarded jointly by Eppendorf and the journal Science. All young scientists who are 35 years of age or younger and who have made outstanding contributions to neurobiological research are invited to apply. The 2013 winner of the Prize will be announced in the fall. The next deadline for applications is June 15, 2014.
For more information, visit www.eppendorf.com/prize.
Eppendorf µCuvette® G1.0 wins red dot design award and innovation prize at Forum Labo
Hamburg, July 2013
The µCuvette G1.0 by Eppendorf, available worldwide since early 2013, has been honored with two awards. On July 1st it was awarded the coveted red dot design award in the category “product design”, presented each year to well thought-out and innovatively designed products. As early as June, the µCuvette G1.0 had been awarded the innovation prize in the category “miniaturization”, by clients as well as by the professional jury, at the international biotechnology exhibition Forum Labo in Paris.
The µCuvette G1.0 is a microliter measurement cell, especially equipped for photometric determination of high concentrations in the smallest of volumes (> 1.5µL). It features a path length of only 1 mm, which corresponds to a 10-fold shorter light path compared to standard cuvettes. Nucleic acid concentrations, for example, can be measured reproducibly at a significantly elevated concentration range. As an accessory to the Eppendorf BioPhotometers and BioSpectrometers, the µCuvette G1.0 thus opens the application range of these instruments to the microliter scale.
“The coated aluminum housing framework and the innovative low-backlash bearing joint of the cuvette convey a high level of robustness and security” states the jury of the red dot design award. Dr. Tanja Musiol, Global Product Manager Detection at Eppendorf AG, is pleased by the awards: “Both prizes show that design and innovation can go hand in hand, and that innovative design has made its mark on the laboratory. The concept of miniaturization is also gaining in significance. Laboratory space is limited; small instruments with accessories capable of expanding their functionality thus provide flexible and space saving solutions.”
Eppendorf has in the past been awarded several previous design prizes for their innovative, high-quality laboratory products. This tradition has now been continued with the red dot design award 2013. For further information, please visit www.eppendorf.com/cuvette and http://red-dot.de