New Eppendorf Tube 5.0 mL system fills the volume gap in sample preparation and storage
When working with samples between 2 and 5 mL, researchers often have no choice but to use tubes that are really designed for larger volumes, typically 15 mL conical screw cap tubes. The new Eppendorf Tube 5.0 mL now provides the ‘missing link’ for such samples, delivering a high-quality solution for volumes up to 5 mL. These new tubes feature a practical and convenient snap cap for single-hand operation and a compact conical design, removing the contamination risks associated with manipulating small volumes in large tubes.
Eppendorf Tubes 5.0 mL are designed for safe and stable centrifugation up to 25.000 x g, eliminating the risk of sample loss when using rapid protocols. Protein LoBind and DNA LoBind variants of the tubes further reduce sample loss by minimising surface binding of the samples, supporting increased yields when used in DNA and Protein isolation. This extra security makes Eppendorf Tubes 5.0 mL highly suited for situations where there is a limited amount of source material. A new polypropylene material provides high transparency and minimizes leaching for safe and reproducible assay data.
The unique sample handling system created by Eppendorf Tubes 5.0 mL has a comprehensive line of accessories for centrifugation, heating, mixing, liquid handling and storage. This allows easy and quick integration into existing workflows across a variety of applications, including cell culture work, DNA purification and sample storage. The Eppendorf Tubes 5.0 mL and the Tube Rack 5.0 mL were awarded with the red dot design award 2013 that recognizes high product quality.
For more information on the new Eppendorf Tube 5.0 mL sample handling system, visit www.eppendorf.com/5mL
Presentation of the 2013 Eppendorf Award for Young European Investigatorto Ben Lehner
This year’s research prize awarded by Eppendorf goes to Spain
Hamburg, June 2013
The British scientist Ben Lehner, Ph.D., ICREA Research Professor, EMBL-CRG Systems Biology Unit atthe Centre de RegulacióGenòmica,in Barcelona, Spain, has won the 2013 Eppendorf Young Investigator Award.
Ben Lehner, born 1978, receives the Eppendorf Award for his discoveries concerning the fundamental question why mutations in the genome result in variable phenotypes. His work has contributed to the understanding how the function of genes is modulated during development by environmental factors and by the interplay with other gene products. The results obtained by Ben Lehner are ground-breaking and offer novel approaches towards the understanding of genetic predisposition for diseases, particularly cancer. The insights obtained by his work are likely to lead to the development of novel therapeutic strategies.
With the 15,000 Euro Eppendorf Young Investigator Award which was established in 1995, Eppendorf AG honors outstanding work in biomedical research and supports young scientists in Europe up to the age of 35. The Eppendorf Award is presented in partnership with the scientific journal Nature.
The Award winner is selected by an independent committee composed of Prof. ReinhardJahn (Chair), Prof. Hans Clevers, Prof. Dieter Häussinger, Prof. Maria Leptin and Prof. Martin J. Lohse.
The official Award ceremony took place at the EMBL Advanced Training Centre in Heidelberg, Germany, on June 6, 2013. Dr. Axel Jahns (Vice President Global e-Business & Marketing Support of Eppendorf AG) and Prof. Matthias Hentze (Associate Director EMBL) welcomed the audience from the scientific community, related industry and the press. The laudatio honoring Ben Lehner’s achievements was held by Prof. Reinhard Jahn (Director of the Max Planck Institute for Biophysical Chemistry in Göttingen, Germany).
“Individual patients do not want to know the possible outcomes of mutations that they carry; they want to know what will actually happen to them. We use model organisms (yeast, worms and tumours) to understand when you can, and why you often cannot, accurately predict the characteristics of individuals from their genome sequences alone”, explained Ben Lehner. “This Award is a testament to the commitment and energy of the past and present members of my laboratory and to our colleagues and collaborators in Barcelona and around the world. Individuals are thankfully all different. One of the main goals of our research is to understand where these differences arise and how they can be predicted”.
More information at www.eppendorf.com/award.
The new generation of Eppendorf ThermoMixer® and ThermoStat incubator raise sample preparationto the next level
Hamburg, May 2013
Ever since the launch of the company’s first performance-defining Mixer and the first Thermostat in 1964 Eppendorf has been at the forefront of mixing and temperature control technology for mL volumes. Thanks to Eppendorf’s continuous development, an exciting new generation of Temperature Control and Mixing Instruments is now set to impress with excellent mixing results, outstanding temperature management and the ergonomics and simplicity essential for routine use in a busy laboratory.
The excellent mixing results of the new Eppendorf ThermoMixer® C, F1.5 and FP (two-in one instruments for combined mixing and incubating) are due to the unique 2DMix-Control technology which ensures fast and efficient mixing in just seconds and now offers reliable mixing solutions in 96- and 384 well plates. Controlled circular movements achieve thorough mixing of liquids in even the smallest volumes, while the anti-spill technology prevents lid wetting and cross-contamination.
For dry incubation, the new Eppendorf ThermoMixer® C and ThermoStat C are operated together with Eppendorf SmartBlocks which are easily exchangeable in just seconds, thanks to the unique Eppendorf QuickReleaseTM technology. These individually sensor-controlled SmartBlocks™ are the best solution for maintaining accurate, uniform temperatures. A wide variety of Eppendorf SmartBlocks support a broad range of applications and temperature-sensitive assays.
For best temperature control performance ever, Eppendorf also launches the unique Eppendorf ThermoTop®, a heated lid with condens.protect technology. This easy to use ThermoTop reliably prevents condensation formation on the tube lid and wall, and in addition supports temperature homogeneity of the thermoblocks to ensure excellent reaction conditions. Simple, wireless handling and its automatic recognition render the Eppendorf ThermoTop® indispensable for optimal performance.
Predefined program and temperature keys provide fast and easy access to the most frequently used mixing/temperature parameters. With simplicity and ease of use at the heart the new Eppendorf ThermoMixer® and ThermoStat instruments contribute to time-saving, stress-free operation and a pleasant working environment.
For more information on the Eppendorf ThermoMixer® family please visit www.eppendorf.com/thermomixer-c.
New Eppendorf PlateReader AF2200 simplifies set-up and analysis for UV/Vis and fluorescence measurements
Hamburg, May 2013
The new Eppendorf PlateReader AF2200 is a highly user-friendly device for quantifying biomolecules, such as nucleic acids and proteins, and for measuring fluorescence-based assays in plate format and is designed to simplify both set-up procedures and data analysis. The AF2200’s convenient hardware and software solutions are engineered for ease-of-use and include integrated temperature control and plate-shaking options, as well as pre-programmed methods incl. data analysis and factor-based calculation. By supporting the instrument with smart accessories, such as the Eppendorf µPlate G0.5 for microvolume applications and pre-configurated filter slides, Eppendorf delivers a complete solution for life scientists across many applications. It is especially valuable where analysis is in the UV/Vis range and/or involves a wide range of fluorescence dyes.
“The software of the PlateReader AF2200 provides the user with pre-programmed applications and a flexible selection of individual parameters,” says Dr Tanja Musiol, Global Product Manager Detection at Eppendorf AG. “It also offers the option to freely program all parameters in order to meet individual requirements. Our pre-configured filter slides are optimized to the pre-programmed methods and are designed to offer the highest flexibility while preventing cross talk. The whole system is developed to simplify set-up procedures and data analysis - making daily lab work easier.”
The broad range of accessories that accompanies the PlateReader AF2200 includes pre-configured filter slides with optimized filter assignment. A choice of 70 additional filters is also available to enable users to tailor measurements for highly specific applications.
For more information about Eppendorf’s PlateReader AF2200 and all the associated accessories, please visit http://www.eppendorf.com/detection.
Eppendorf launches the new electronic pipette controller Easypet® 3
Hamburg, April 2013
Eppendorf has launched the new aid for electronic pipetting, Easypet 3. Replacing the existing model, the new model gives greater accuracy by controlling the speed conveniently and intuitively with the use of the operating buttons. The new Easypet 3 boasts decreased battery charging time to 3 hours, allowing for increased cordless operation time. With its lightweight ergonomic design Easypet 3 enables stress-free pipetting.
Easypet 3 promotes reliability, ease of use and simplicity. Operation buttons are ergonomically shaped, making aspirating and dispensing of liquids easier. Increased or reduced pressure on the buttons regulates speed for precise measurements. Vibrant backlit LEDs highlight the battery status contributing to the user’s peace of mind. Easypet 3 delivers efficient performance through a high-powered motor speeding up the pipetting process and can be used with any type of pipettes from volumes of 0.1 – 100 mL.
Easypet 3 is also able to be operated while recharging, ensuring that the workflow is not interrupted. Easypet 3 has been designed with the user in mind. It is lightweight, well-balanced and extremely easy to use. Being cordless, it enables flexibility in the laboratory allowing the user to freely move around the workplace.
The ergonomic design includes a hand rest and wall mount that support long-term use.
The Easypet 3 was awarded with the red dot design award 2013 that recognizes high quality. Eppendorf has already won several design awards for its innovative, top-quality lab products in the past. The red dot product design award 2013 continues this tradition.
For more information on Easypet 3 contact your local distributor or visit www.eppendorf.com/easypet.
Eppendorf® introduces a single-use bioreactor for microbial applications
Juelich/Hamburg, March 26th, 2013 –
With the introduction of the new BioBLU® 0.3f, Eppendorf® offers for the first time a fully-instrumented single-use bioreactor designed specifically for microbial applications. The BioBLU 0.3f joins the BioBLU 0.3c which was presented last year and is designed for the cultivation of animal and human cells. Both the BioBLU 0.3c and BioBLU 0.3f are specifically designed for use with the compact DASbox® mini bioreactor system. Eppendorf’s DASbox system is a unique mini bioreactor system for parallel operation of 4, 8 or more mini-bioreactors and well suited for Design of Experiment (DoE), bioprocess development screening and for use as a scaledown model. With the new BioBLU 0.3f, Eppendorf is paving the way for users of conventional microbial-based biotechnology to take advantage of, the time and cost benefits of single-use bioreactor technology, such as in pharmaceutical product development.
The fully-instrumented BioBLU 0.3f single-use bioreactor features working volumes of 65 to 250 mL and meets all requirements for microbial applications. The rigid-wall, single-use bioreactor includes two Rushton impellers achieving oxygen intake levels comparable to industrial glass and stainless steel bioreactors. All critical parameters, such as temperature, pH and dissolved oxygen, can be monitored and regulated using industry standard sensors. Integrated dip tubes permit the controlled feeding of liquids, easy sampling and massflow-controlled gassing. The specially developed, magnetically-coupled overhead drive safely operates up to 2,000 rpm, ensuring that the BioBLU 0.3f supports the high agitation rates required for microbial applications. The innovative liquid-free Peltier technology ensures that the temperature of the culture is precisely controlled, even with modern high cell density processes that generate extraordinarily high levels of heat. The integrated Eppendorf technologies represent another milestone in porting the requirements of conventional bioreactor technology to single-use systems. All wetted materials in the BioBLU 0.3f are USP Class VI certified and conform to United States Food and Drug Administration requirements.
The development of the BioBLU 0.3f single-use bioreactor for microbial applications once again highlights the strong bioprocessing synergies that exist between Eppendorf, New Brunswick™ and DASGIP®, which was incorporated into the Eppendorf family in early 2012. Combining Eppendorf's expertise in polymer processing with DASGIP's engineering ingenuity in bioreactor design has allowed Eppendorf to expand its single-use bioreactor portfolio for microbial applications.
"We're pleased to be taking a new direction in single-use bioreactor technologies," says Dr. Matthias Arnold, Managing Director of DASGIP Information and Process Technology GmbH. "The physical requirements placed on single-use systems for microbial applications is much higher than the demands that are placed on cell culture processes, the conventional field of application for disposable bioreactors. By working together with the polymer experts at Eppendorf, we managed to overcome these hurdles, and as a result were able to optimally match material, design and functionality at the highest level of quality."
Additional information can be found at: eppendorf.com/dasgip
Claudia M. Hüther-Franken
Eppendorf AG Bioprocess Center Europe,
Tel: +49 2461 980 -471, firstname.lastname@example.org
Eppendorf posts sales in excess of 500 million euros for the first time
- Sales grow by 8.9 percent
- EBIT above €100 million
- Expansion of product portfolio and sales footprint remains a focus in 2013
Hamburg, March 25, 2013 – The Eppendorf Group performed well in fiscal year 2012, generating sales of more than €500 million for the first time. Sales increased by 8.9 percent year on year to €520.2 million (prior year: €477.7 million). Organic growth, after adjustment for currency effects and acquisitions, was 4.0 percent. Income from operations (EBIT) improved by 11.5 percent to €101.5 million (prior year: €91 million). Net income in 2012 rose to €70.6 million, which is 14 percent over fiscal 2011. In order to keep on developing its product portfolio, the Group increased its investments in research and development in 2012 by 10.7 percent to a total of €27.5 million (prior year: €24.9 million). Eppendorf also invested in expanding its international sales footprint and opened new sales companies in Korea and Russia.
“In view of the challenging market environment for our industry, we can be satisfied with how we did in fiscal 2012,” said Dr. Dirk Ehlers, CEO of Eppendorf AG. “In 2012 we expanded our Cell Handling application area by acquiring the DASGIP Group, an important addition for us. Continuous development of our product portfolio, whether through research and development or through acquisitions, will remain a focus of our activities in 2013,” added Ehlers.
Eppendorf extended its detection product portfolio by launching new instruments and accessories
Hamburg, March 2013
With the addition of the new BioSpectrometer fluorescence and the new BioPhotometer D30, Eppendorf has extended its family of detection instruments. The new instruments offer additional measurement capabilities and features to the wide range of Eppendorf´s easy-to-use instruments that combine effective hardware with powerful and innovative software solutions. The detection instrument portfolio of Eppendorf is supported by accessories and consumables, such as the UVette®, a UV-transparent disposable cuvette, and the new semi-micro and macro Vis Cuvettes. Additional accessories that include reference filter sets and a microvolume measurement cell, the µCuvette G1.0, to measure sample volumes of only 1.5µL, are available.
“Eppendorf continues a longstanding tradition with over 60 years of experience in the field of photometry.” said Dr. Tanja Musiol, Global Product Manager Detection at Eppendorf AG. “From the very beginning, the focus was on obtaining maximum benefits while using the simplest handling methods. This philosophy has remained unchanged to this day. With the current product portfolio, Eppendorf allows to select the optimal combination of devices and accessories from a large, rapidly expanding, range of products. From fluorescence-based applications to microvolume measurements: anything is possible!”
The Eppendorf detection family is used in a broad range of life science applications, including kinetic and fluorescence measurements as well as microvolume measurements. The combination of Eppendorf instruments, accessories and consumables enables users to perform many different applications, with variable sample volumes and concentrations in a flexible way. Equipment can be selected and combined to match the specific requirements of the work taking place, while achieving highest reliability.
For more information regarding the Eppendorf detection portfolio, please visit www.eppendorf.com/photometry.
Automated TruSeq® Stranded Total RNA Library Preparation Method for Eppendorf epMotion
Hamburg, February, 2014
Today Eppendorf announces the release of the epMotion automation method for Illumina’s TruSeq Stranded Total RNA library preparation kit for next-generation sequencing as “Illumina Qualified.” “Illumina Qualified” indicates that Illumina’s analysis of libraries prepared with this epMotion method has shown the libraries to perform comparably to those prepared manually.
Sample preparation for next-generation sequencing (NGS) involves extensive manual processing that can affect reproducibility. Eppendorf epMotion Liquid Handling systems are important tools for labs that want to significantly increase throughput and consistency of results. The automation of labor-intensive steps in NGS sample prep, like pipetting, mixing, temperature control and magnetic separation, brings about a standardized and streamlined process, with significantly reduced hands-on time and minimization of human errors.
“Eppendorf’s method for TruSeq Stranded Total RNA provides our customers with another great option for high throughput automation,” said Peter Fromen, Senior Director of Product Marketing at Illumina. “Eppendorf is one of the leading providers of automation to NGS users, and we are looking forward to additional methods from them in the near future.”
“The Stranded Total RNA sequencing library preparation using the epMotion minimizes our hands-on time while achieving overall high reproducibility. This streamlines and standardizes our sequencing workflow, thereby allowing for higher sequencing throughput. We are looking forward to collaborating with Eppendorf on additional automated library prep protocols,” said Dr. Wei Chen, Head of the Scientific Genomics Platform at Max Delbrück Centre for Molecular Medicine in Berlin, Germany.
“This method is tailored for 8, 16 or 24 samples of 100 to 1000 ng total RNA and gives sequencing ready libraries in 9 to 12 hours. While this is not much faster than the manual process, the hands-on time is significantly reduced as the automated process needs less than one hour preparation time in total,” said Dr. Reza Hashemi, Executive Director of Portfolio Management Liquid Handling at Eppendorf.
Additional automated methods for NGS library preparation from Illumina are currently in development.
Eppendorf’s new epMotion® systems reliably undertake even more liquid handling tasks
Eppendorf has launched seven new models to its epMotion range of automated pipetting systems. Designed for effective, automated liquid handling applications, the new epMotion 5070 and epMotion 5075 systems provide a precise, accurate alternative to time-consuming manual pipetting and liquid handling tasks. The combination of accelerated operational speed, new software & hardware features and an increased number of worktable positions make the systems ideal for simple and flexible laboratory use across a wide range of liquid handling applications.
To ensure easy and consistent use, the epMotion 5070 and epMotion 5075 employ both Eppendorf’s established epBlue™ software and an intuitive new software assistant concept. This supports the simple programming of applications and ensures that all users can use the system with only initial training. In addition, novel features such as a new CleanCap option, including UV light decontamination and a HEPA filter for purified air (available for the larger 5075 systems), offer new standards of decontamination and sample safety. This is supported even further by an optical sensor that checks labware, liquid levels and tip counts and type at the start of each cycle, hugely decreasing any risk of error during the run.
“The need for flexible, easy automation of liquid handling tasks is well known,” commented Dr Carsten Buhlmann, Global Product Manager for Automation at Eppendorf AG. “The specialized robotic system in the epMotion 5070 and epMotion 5075, along with the increase to 15 worktable positions, provides even greater application flexibility. More applications can be carried out rapidly and efficiently, and the innovative range of features, including the CleanCap option and software assistants, ensures that users can work with complete confidence.”
Also available is the new epMotion 5075m, which combines magnetic bead separation abilities with the rest of Eppendorf’s innovative technologies to create the most flexible system in the epMotion range.
For more information on Eppendorf’s new epMotion systems, please visit www.eppendorf.com.