Eppendorf introduces first ready-to-use, synthetic cell cultureware for iPSC and MSC stem cells
Since the groundbreaking discovery of human induced pluripotent stem cells (hiPSCs) by Shinya Yamanaka in 2006, the use of pluripotent stem cells (PSCs) has experienced a boom. Due to their extensive in vitro self-renewal properties and their ability to differentiate into many different cell types, PSCs, and especially hiPSCs, offer exciting promises in a wide range of cell applications including regenerative medicine.
The major challenge of iPSC-cultivation is to preserve their properties until differentiation is induced. To support this, an appropriate culture medium and a defined extracellular matrix-mimicking growth surface/coating are crucial. Biological coatings commonly used to expand stem cells are inherently complex, non-defined growth surfaces that frequently reduce experimental reproducibility. This is exacerbated by lot-to-lot variations between coating media, by the use of undefined growth factors and extracellular matrix (ECM) components known to sustain cell adhesion and pluripotency, as well as by a potential pathogen contamination risk during preparation and storage. Therefore, in order to provide defined conditions, fully synthetic, animal component/human component-free culture systems are of great interest.
However, in contrast to other types of stem cells, synthetic and ready-to-use growth surfaces are currently not available for human induced PSCs (hiPSCs). Eppendorf has now introduced a ready-to-use surface with synthetic fibronectin-derived motifs to support cell attachment by mimicking native ECM proteins. According to the manufacturer, this novel surface supports long-term expansion of hiPSCs over 25 passages and is also suitable for hMSCs and other ECM-dependent cells. Furthermore, it allows expansion of stem cells in xeno-free and restrictive culture conditions, aiming to provide a completely defined culture system for PSCs without animal or human components.
Information about Eppendorf CCCadvanced™ FN 1 motifs cell cultureware, including detailed expansion analyses of hiPSCs and hMSCs, is available at www.eppendorf.com/ccc-advanced
2018 Eppendorf Award for Young European Investigators presented to Andrea Ablasser
Heidelberg/Hamburg, June 22, 2018
In 2018, the Hamburg life science company is presenting its highly prestigious research prize for the 23rd time. The independent Eppendorf Award Jury chaired by Prof. Reinhard Jahn selected Prof. Dr. Andrea Ablasser, Assistant Professor at the Swiss Federal Institute of Technology, Lausanne, Switzerland, as the 2018 winner of the Eppendorf Award for Young European Investigators.
The Award ceremony took place at the EMBL Advanced Training Centre in Heidelberg, Germany, on June 21, 2018. The laudatio honoring Andrea Ablasser’s achievements was held by Award Jury Chairman Prof. Reinhard Jahn of the Max Planck Institute for Biophysical Chemistry, Göttingen.
Andrea Ablasser, born 1983, receives the € 20,000 prize for her contributions to a key step in the innate immune response, which triggers a frontline defense when cells are attacked by microorganisms. The Jury: “Andrea Ablasser’s work sheds light on the mechanisms by which other cells are informed about the presence of foreign DNA, and she has recently shown that the same pathway can be triggered in ageing cells, contributing to senescence. This work may lead to novel therapeutic approaches for both microbial infections and autoinflammatory syndromes."
Andrea Ablasser: “I am greatly honored to receive the prestigious Eppendorf Award. It recognizes the contributions of several gifted colleagues and students, who all took part in this long, but exciting research effort. We are fascinated by the fundamental mechanisms of innate immune recognition that enable defense against pathogens and hope that our research will in the future aid the development of new treatment options for human diseases.”
With the Eppendorf Young Investigator Award, which was established in 1995, Eppendorf AG honors outstanding work in biomedical research and supports young scientists in Europe up to the age of 35. The Eppendorf Award is presented in partnership with the scientific journal Nature. The Award winner is selected by an independent committee composed of Prof. Reinhard Jahn (Max Planck Institute for Biophysical Chemistry, Göttingen, Germany), Prof. Dieter Häussinger (Clinic for Gastroenterology, Hepatology and Infectiology, Düsseldorf, Germany), Prof. Maria Leptin (EMBO, Heidelberg, Germany), Prof. Martin J. Lohse (Max Delbrück Center for Molecular Medicine in the Helmholtz Association, Berlin, Germany), and Prof. Laura Machesky (Cancer Research UK Beatson Institute, Glasgow, UK).
More information about entry details, judging procedures, and past winners can be found at www.eppendorf.com/award
The new 15 mL and 50 mL Eppendorf Conical Tubes - Protein LoBind and Amber
Hamburg, June 2018
The latest major innovation for the product is the introduction of these two Eppendorf Conical Tube versions.
The Protein LoBind Conical Tubes minimize the risk of non-specific protein and peptide samples sticking to the walls of the tubes. This ensures the best possible sample recovery, which is especially important when working with samples with low initial concentration.
The innovative material of the amber-colored Conical Tubes is highly transparent, while at the same time effectively protecting the sample from low-wavelength light. This way, the pipette tip immersion can be safely monitored during liquid transfer, which helps avoid cross-contamination.
Further information can be found at: www.eppendorf.com/conicals
Introduction of the Eppendorf Fast PCR Tube Strips
Hamburg, June 2018
Eppendorf is a renowned innovator in the field of PCR Instruments and consumables. Now Eppendorf extends its range of PCR consumables with a new innovation, the Fast PCR Tube Strip. Scientists are able to easily achieve visibly better yield using the Fast PCR Tube Strip combined with fast reagents and a fast cycler, such as the Mastercycler X50.
The Eppendorf Fast PCR Tube Strip has a higher thermal conductivity than polypropylene, allowing the heat from the PCR block to transfer to the reaction quicker than before. This allows for better control over the reaction parameters and achieves better results. The days of reducing reaction volumes or sourcing thin walled tubes to address Fast PCR are gone!
As mentioned, the new Fast PCR Tube Strip is particularly well suited to users of PCR cyclers with high ramp rates or to users of fast enzymes or reagent kits.The effect is achieved by utilising the natural properties of polyethylene. This has been accomplished whilst maintaining the format of the traditional polypropylene PCR vessel, allowing scientists to easily transfer from their current polypropylene PCR tube strips to the new Fast PCR Tube Strip without having to invest in specialised equipment.
The new Eppendorf Fast PCR Tube Strip is suitable for end-point PCR and is available in three variant packs, with domed cap strips, with flat cap strips or without caps.
Eppendorf commissions fully automated high-bay storage in Oldenburg in Holstein
Hamburg, June 1, 2018 – With the commissioning of its fully automated high-bay storage facility, Eppendorf is safeguarding the long-term vitality of its production site in Oldenburg in the German state of Schleswig-Holstein. This major investment in new construction will enable Eppendorf to improve product availability and its logistic processes to meet increased customer demand.
The high-bay storage facility ensures optimized warehousing as part of Eppendorf’s overall competence center for research, development and the production of laboratory consumables. In the process, the functions of production and logistics will be aligned more closely under the aspect of customer orientation, so that the company can continue to reliably supply its customers around the world.
Dr. Wilhelm Plüster, Member of the Management Board and Chief Technology Officer Eppendorf AG, says: “Eppendorf is a company that enjoys sustainable above-average market growth. The commissioning of this new high-bay storage facility enables us to continue our tradition of professional warehousing and thus ensure the optimal availability of our products.”
The high-bay storage facility went into operation at the dedication ceremony on June 1, 2018. The facility is 100 meters long, 30 meters wide and 32 meters high, and has more than 13,000 pallet bays, offering optimized warehousing capacity for the laboratory consumables produced on-site.
This long-term investment reinforces the importance of the Oldenburg location and its development, and is part of the Eppendorf Group’s orientation toward company development aimed at sustainable, continued success.
Ready-to-use, synthetic surface cultureware for human induced pluripotent stem cells (hiPSCs) coming to market
Hamburg, April 2018
The major aim during the multi-passage cultivation of pluripotent stem cells (PSCs) is to preserve their differentiation and growth potential. Here, the proper selection of a defined culture system that consists of a growth surface and culture medium is crucial. The commonly used biological coating materials for PSC expansion are based on either mouse embryonic fibroblast feeder layers or animal-derived protein mixtures. However, these coating materials represent complex and non-defined growth surfaces that frequently lead to an insufficient experimental reproducibility. This disadvantage is exacerbated by variable lot-to-lot quality and purity of coating media, by use of undefined growth factors and extracellular matrix (ECM) components known to sustain cell adhesion and pluripotency, as well as by a potential pathogen contamination risk during preparation and storage. Therefore, to ensure robust cell performances in downstream applications, fully synthetic, animal-component-free culture systems have always been of great interest in this context.
In contrast to other types of stem cells, synthetic and ready-to-use culture systems are currently not available for human induced PSCs (hiPSCs). In June 2018, Eppendorf will bring a novel, synthetic and ready-to-use surface for hiPSCs, hMSCs and other ECM-dependent cells to the market. This surface is made up of fibronectin-derived motifs to support cell attachment by mimicking native ECM proteins. Furthermore, it allows expansion of stem cells in xeno-free and restrictive culture conditions, aiming to provide a completely defined culture system for PSCs without animal or human components. According to the manufacturer, this novel surface supports consistent and robust growth rate of hiPSCs with all pluripotent-specific characteristics over at least 20 passages.
Eppendorf CCCadvanced™ FN 1 motifs cell culture plates and flasks will be introduced to the scientific community at the ISCT conference in Montreal. Additional information on detailed expansion analysis is available at http://www.eppendorf.com/appnote389 and http://www.eppendorf.com/appnote390
Fiscal year 2017: Eppendorf continues to generate growth above the market average
- Group sales of €690.3 million with organic growth of 7.9% in local currencies
- Income from operations increased to €145.5 million; EBIT margin of 21.1%
- Stronger strategic investments for further sustainable growth
Hamburg, April 24, 2018 – The Eppendorf Group can look back at 2017 as a very satisfactory business year. With Group sales of €690.3 million (prior year: €651.5 million), the company generated growth of 6.0% (organic growth in local currencies: +7.9%) while the world market grew by around 5%. As expected, income from operations (EBIT) developed at a slower rate than sales as a result of stronger strategic investments, rising by just 4.2% to €145.5 million (prior year: €139.6 million). This corresponds to an EBIT margin of 21.2% (prior year: 21.4%).
All market regions and all major product groups contributed to Eppendorf’s business success in the reporting year. In North and South America, the growth momentum accelerated once again. As far as Europe is concerned, the greatest contribution to the increase in sales came from double-digit growth rates in Eastern Europe. Meanwhile the momentum in the Asia/Pacific/Africa region was driven mainly by growth in the key market of China.
Thomas Bachmann, President and Chief Executive Officer of Eppendorf AG, comments: “Eppendorf’s excellent business and strong market position in 2017 offered ideal conditions to set up the company for continued successful development in the future. In parallel with our operating business, we worked intensively on various strategic initiatives and projects, especially in the areas of market presence, innovation and corporate culture.”
For instance, Eppendorf established a new global organization – effective as of January 1, 2018 – to focus itself even more strongly on the needs of the market. These organizational changes are closely linked to a comprehensive innovation initiative that aims to bring products onto the market as quickly as possible. As in the year before, Eppendorf increased its research and development expenditures considerably: In 2017, they amounted to €36.5 million compared with €34.8 million in the previous year. The roundabout twenty new products that the company launched in 2017 already made an important contribution to its growth.
Eppendorf’s aspiration for 2018 is to consistently and sustainably implement the projects it has launched for the further development of the company. It has also budgeted further substantial investments in innovation, organization, infrastructure and production capacities as well as training and continuing education. The company considers itself in a good starting position for another successful business year.
You can find Eppendorf AG’s 2017 Annual Report at
Eppendorf is a leading life science company that develops and sells instruments, consumables, and services for liquid handling, sample handling, and cell handling in laboratories worldwide. Its product range includes pipettes and automated pipetting systems, dispensers, centrifuges, mixers, spectrometers, and DNA amplification equipment as well as ultra-low temperature freezers, fermentors, bioreactors, CO2 incubators, shakers, and cell manipulation systems. Consumables such as pipette tips, test tubes, microliter plates, and single-use bioreactor vessels complement the range of highest-quality premium products.
Eppendorf products are most broadly used in academic and commercial research laboratories, e.g., in companies from the pharmaceutical and biotechnological as well as the chemical and food industries. They are also aimed at clinical and environmental analysis laboratories, forensics, and at industrial laboratories performing process analysis, production, and quality assurance. Eppendorf was founded in Hamburg, Germany in 1945 and has more than 3,100 employees worldwide. The company has subsidiaries in 26 countries and is represented in all other markets by distributors.
Introduction of the epMotion® 5075 NGS solution for automated library preparation
Hamburg, March 2018
The Next Generation Sequencing (NGS) library preparation process is labor-intensive, requiring experience and attention to detail over hours. Eppendorf is introducing a complete package of epMotion 5075 automated liquid handling workstation with all required accessories, a broad range of vendor specific pre-developed & qualified methods, advantageous consumables and related service products that are tailored for the automation of the NGS library preparation to increase the efficiency and ease of use in your lab. The epMotion 5075 NGS solution comes with a new set of software features that was specifically developed for NGS customer needs, like intelligent selection of single and 8-channel dispensing tools, flexible number of samples, reduced dead-volume, more worktable capacity due to improved labware stacking, email notification and more, and of course include Eppendorf DNA LoBind consumables in this starter package.
“With the new solution package and features our epMotion 5075t NGS system will be positioned as the preferred solution for small to medium sample throughput with maximum flexibility for varying daily sample numbers and highest flexibility for a vast list of NGS library preparation kit methods to automate” said Dr. Jochen Mueller-Ibeler, Head of Business Unit Automated Liquid Handling at Eppendorf.
New development in the field of Liquid Handling: Highly viscous substances such as collagen are now pipettable
Hamburg, March 2018
Handling of volumes in the mL range of highly viscous liquids such as 99 % glycerol, detergents or collagen in the laboratory and for analytical purposes has proven difficult, requiring the use of suboptimal tools such as spoons. These requirements cause tremendous delays to analysis and mixing procedures. As such, the precise volume of the liquid must frequently be determined via several material-intensive steps: in an initial time-consuming step, the highly viscous liquids are transferred between different vessels, followed by weighing and calculating the volume by consulting the liquid’s specific density.
In order to process aqueous liquids in precisely, modern laboratories also use pipettes with different functional principles, each equipped with the respective consumable tips. For viscous liquids in particular, those pipettes that function according to the direct displacement principle, similar to that of a syringe, have prevailed. However, increasing viscosity leads to growing flow resistance inside the tip. The consequence: either the hand strength of the operator, the material comprising the operational controls or the motors of electronic pipettes will reach their limit. Additionaly, the processing speed will decrease with increasing viscosity, particularly if precision is of the essence. Up to now, the limit of the dynamic viscosity – which is dependent on the capacity of the pipetting system or the operator, respectively – consistently ranged between approximately 200 and 300 mPa*s (10 mL tip volume). This is roughly equivalent to the viscosity of 86 % glycerol at 20 °C.
A new invention by the company Eppendorf, which has been developing Liquid Handling Instruments for the past 57 years, is about to dissolve this limit. The new ViscoTip® for the associated Multipette instrument is purportedly capable of processing tough liquids such as collagen with viscosities of up to 14,000 mPa*s precisely and quickly. It will be introduced to the public for the first time in April 2018 at the analytica exhibition. Additional information, including information on the liquids tested, is available at www.eppendorf.com/viscotip
Eppendorf – A New Spin on Centrifugation
Eppendorf started off the year by introducing not one, but two new centrifuges: A generation change at the small 24-place benchtop centrifuges. In the segment of the large refrigerated benchtop centrifuges Eppendorf is expanding the product range with a particularly versatile, high-capacity variant.
The new Centrifuge 5425 holds up to 24 vessels with a volume of 1.5 or 2.0 mL. A new swing-out rotor for 96 PCR tubes broadens the spectrum of molecular applications. Harvesting bacteria and yeast cells is facilitated by a new fixed-angle rotor for 5 mL tubes. The Centrifuge 5425 replaces the legendary Centrifuge 5424; it scores with a whole string of improvements, the highlights of which include certified aerosol-tight Eppendorf QuickLock® rotor lids for simple and safe handling.
The new Centrifuge 5910 R convinces with its versatility and capacity. Cell harvests carried out in bottles up to 750 mL, large scale purification of DNA/RNA as well as Ficoll® gradients are just a few examples of the many areas of application that are served by this new centrifuge. The new universal adapters facilitate even quicker loading; using these new adapters, conical vessels of up to 50 mL, plates and 250 mL bottles may be centrifuged for the very first time without the need for changing rotor, rotor buckets or adapters.
For more information, please visit:
Centrifuge 5425: www.eppendorf.com/my-lab-my-centrifuge
Centrifuge 5910 R: www.eppendorf.com/next-benchmark