Eppendorf's new HeatSealers are small, reliable and flexible
By launching the new HeatSealer Family Eppendorf offers now two new devices for reliable sealing of different plate formats. To match various requirements, there is a choice between a small and extremely user-friendly device, HeatSealer S100, and a slightly bigger version, HeatSealer S200, with flexible programmable sealing parameters. Both models allow for easy, reproducible and effective sealing of plates, thereby efficiently preventing evaporation and contamination.
Thanks to a selection of adapters the standard plate formats can still be used. Whether hermetic sealing of multiwell plates or effective evaporation protection for low-profile PCR plates is required, the Eppendorf HeatSealer is always the right choice. An integrated thermostat prevents overheating while enabling reliable and reproducible sealing of the plate. The press-on mechanism was improved and makes handling even more simple. Furthermore, the HeatSealer S200 features different sealing temperatures and times, selectable depending on requirements, plates and sealing option.
„A reliable assay result can only be achieved with plates that are reproducibly and tightly sealed. This also applies to the storage of samples. Especially for long-term storage, tight sealing is crucial for save storage of your sample until you need it again!“ says Dr. Kay Körner, Senior Global Product Manager PCR with Eppendorf.
For more information go to: www.eppendorf.com
Eppendorf and Florida State University Collaboration Demonstrates Large-Scale Stem Cell Expansion
Enfield, CT USA, March 16, 2015 – A collaboration between Eppendorf and Florida State University has successfully shown the feasibility of large-scale expansion of human mesenchymal stem cells (MSC) using the company’s proprietary Fibra-Cel® packed-bed technology.
Dr. Ma Sha, Director of Technical Applications at Eppendorf, provided equipment and funding for reagents to Dr. Teng Ma to support the Florida State team’s work in the field of stem cell expansion. The team successfully conducted MSC expansion inside a packed-bed 3D matrix using a CelliGen® 310 bioreactor at ~2 L scale. The results demonstrate the feasibility of large-scale stem cell expansion using the company’s patented Fibra-Cel packed-bed technology which provides uniform mass transfer of nutrients and oxygen to the adhered cells. The finding will be submitted for publication at a peer reviewed scientific journal.
Dr. Yaw D. Yeboah, Dean and Professor, College of Engineering, thanked Eppendorf and noted that the company’s commitment to the University allows it to continue to deliver world-class education and transformative experiences that prepare students for the challenges of today’s complex, knowledge-driven marketplace.
The new Eppendorf Conical Tubes 15 mL and 50 mL broaden the volume range of Eppendorf Tubes®
Hamburg, February 2015 - With the introduction of the two screw cap tubes 15 mL and 50 mL, Eppendorf Tubes® now cover the entire volume range from 0.5 mL to 50 mL.
The newly designed screw caps provide not only optimal sealing properties, but with their grooved and multi-surface side-contour they simultaneously ensure a secure, slip-free grip. The optimized handling features further facilitate safe opening and closing of the tubes via convenient one-handed operation.
The Eppendorf Conical Tubes are further characterized by an expanded purity grade: they are not only sterile and pyrogen-free, but they are also free from DNases and RNases as well as human and bacterial DNA. These features make them ideally suited for cell biology applications in a sterile environment as well as for laboratory protocols in the fields of microbiology and molecular biology, which rely on freedom from contaminations with DNA.
The highest level of manufacturing precision and robustness ensure smooth performance of these tubes in laboratory instruments such as centrifuges or thermomixers.
“With all these high quality product features, the Eppendorf Conical Tubes 15 mL and 50 mL make a significant contribution to reliable and reproducible results in a broad variety of laboratory protocols”, says Dr. Nils Gerke, Global Product Manager for Consumables at Eppendorf.
More information is available at: www.eppendorf.com/conicals
Eppendorf & Science Prize for Neurobiology 2015 Call for Entries!
Eppendorf and the journal Science are now accepting applications for the 2015 Eppendorf & Science Prize for Neurobiology. This annual international research prize of US$25,000 is awarded to young scientists for their outstanding contributions to neurobiology research based on methods of molecular and cell biology. Researchers who are 35 years of age or younger are invited to apply by June 15, 2015. The winner and finalists are selected by a committee of independent scientists, chaired by Science’s Senior Editor, Dr. Peter Stern.
The 2014 prize was won by the US scientist Eiman Azim, Ph.D., Postdoctoral Research Fellow at Columbia University in New York. Eiman Azim’s work offers fundamental new insights into the neural mechanisms that enable skilled limb movements to be both smooth and precise. His research has provided direct support for long-standing theories about the roles of internal feedback pathways within the central nervous system and external feedback from the muscles in regulating fine motor control.
For more about the prize and film interview with Eiman Azim, go to www.eppendorf.com/prize.
Eppendorf wins its first patent infringement case in China
Hamburg, January 30, 2015 – Eppendorf AG (“Eppendorf”) has successfully enforced its Chinese patent ZL200710079795.X, covering its vortexing technology for mixers used in the Eppendorf MixMate®. Beijing First Intermediate People’s Court issued judgment Yi Zhong Min Chu Zi No. 871 on December 10, 2014, finding that the manufacturer Hangzhou Allsheng Instruments Co., Ltd. (“Allsheng”) and its distributor Beijing Aochuang Industrial Technology Development Co., Ltd. infringed upon the Eppendorf patent. The ruling forbids production, offer and sale of the infringing mixer product by Allsheng and sale and offer of the product by its distributor, and awards Eppendorf monetary damages and compensation for expenses.The ruling is final and in force.
This was the first patent suit initiated by Eppendorf in the People’s Republic of China. “Eppendorf sees this ruling as a confirmation of its patent strategy of filing a substantial number of its inventions for patent protection in China. It is important to see that we are able to successfully enforce intellectual property rights in China.” commented Sven Bülow, Ph.D., Executive Vice President Corporate Development at Eppendorf AG.
Eppendorf Announces New BioFlo® 320 Bench Scale Bioprocess Control Station
Enfield, CT USA, January 5, 2015 – The BioFlo 320 is truly a step into the future for Eppendorf as a bioprocess equipment manufacturer. New features including autoclavable and single-use vessel flexibility, intelligent sensors, and IP network communication for multi-unit control set it apart as the new premium choice in the bench scale bioprocess market.
Suitable for microbial and cell culture, scale up to scale down, batch, fed-batch, and continuous processes, the BioFlo 320 can meet the ever-changing needs of all segments of the biotech and pharmaceutical industries. It offers flexibility, better control, and maximum functionality while occupying a fraction of the valuable lab space of similar systems.
Research on Skilled Limb Movement Wins 2014 Eppendorf & Science Prize
Hamburg, October 2014 - The US scientist Eiman Azim, Ph.D., Postdoctoral Research Fellow at Columbia University in New York has won the 2014 Eppendorf & Science Prize for Neurobiology. Dr. Azim’s work offers fundamental new insights into the neural mechanisms that enable skilled limb movements to be both smooth and precise.
Dr. Azim writes, “When we catch a ball or throw a dart, we usually don’t give a second thought to the intricate neural circuits that orchestrate such dexterous behaviors. Yet for these actions to succeed, the nervous system must continuously tweak motor output to shape and refine movement of the arm. As a postdoctoral fellow with Tom Jessell, I have been using the genetic accessibility of mice to disentangle individual spinal circuits that help achieve the precision and fidelity of reaching movements. By applying mouse molecular tools to the investigation of skilled forelimb behavior, traditionally the domain of primate research, my colleagues and I provide direct experimental support for long-standing theories about how internal feedback pathways within the central nervous system and external feedback from the muscles each contribute to fine motor control. In the future, I plan to build on the approaches we developed to investigate how the cerebellum uses feedback information to refine movement. More generally, I hope to explore how the elaboration of motor circuits across developmental and evolutionary time directs diverse repertoires of skilled motor behavior, with an eye toward a better understanding of human motor function and dysfunction.”
According to Prize Jury Chair and Science Senior Editor, Dr. Peter Stern, “Eiman Azim’s work has furthered our understanding of the neural circuits involved in skilled movement. He established that propriospinal neuron internal feedback and motor copy circuits help to calibrate movement. Dr. Azim identified presynaptic inhibition as a crucial gain control mechanism for smooth limb movement.”
The annual US$25,000 Eppendorf & Science Prize for Neurobiology honors scientists, like Dr. Azim, for their outstanding contributions to neurobiology research. Dr. Azim is the 13th recipient of this international award. The Prize is presented at a ceremony held in conjunction with the Annual Meeting of the Society for Neuroscience. All scientists who are 35 years of age or younger and who have made outstanding contributions to neurobiological research based on methods of molecular and cell biology are invited to apply. The next deadline for applications for the Eppendorf & Science Prize for Neurobiology is June 15, 2015. For more information about Dr. Azim and the Prize, visit www.eppendorf.com/prize.
Eppendorf Award for Young European Investigators 2015: Call for entries!
Hamburg, October 2014 - Until 15 January 2015, young researchers working in Europe who are not older than 35 years are invited to apply for the Eppendorf Award for Young European Investigators. This highly prestigious prize acknowledges outstanding contributions to biomedical research in Europe based on methods of molecular biology, including novel analytical concepts. In 2015 the Eppendorf Award for Young European Investigators celebrates 20 years. To mark this milestone, Eppendorf has increased the prize money to 20,000 euros.
The winner is selected by an independent expert committee chaired by Reinhard Jahn (Max Planck Institute for Biophysical Chemistry, Göttingen, Germany).
The Award winner 2015 will receive
- a prize money of 20,000 EUR
- an invitation to the prize ceremony at the EMBL Advanced Training Centre in Heidelberg, Germany, on June 25, 2015
- an invitation to visit Eppendorf AG in Hamburg, Germany
- coverage of his/her work by Nature in print and online (including a podcast)
Full details on the Eppendorf Award, the selection criteria and past Award winners can be found at www.eppendorf.com/award. Here potential prize winners will find all relevant information for a successful application.
Only online applications are accepted. The official online registration portal will accept applications as of October 1, 2014 at www.eppendorf.com/award/application.
The Eppendorf Award for Young European Investigators is presented in partnership with Nature.
Eppendorf licenses technology for thermal cyclers to SensoQuest
Hamburg, September 15, 2014 – Eppendorf AG has licensed patents covering gradient technology for thermal cyclers to SensoQuest GmbH, including German patent Nos. DE 196 55 282 and DE 196 55 141. Eppendorf utilizes the technology in its Eppendorf Mastercycler® pro gradient and Mastercycler nexus gradient models.
Next Automated NGS Library Preparation Method for the Eppendorf epMotion: TruSeq® Stranded mRNA
Hamburg, September 2014
Today Eppendorf announces the availability of the epMotion automation method for Illumina’s TruSeq Stranded mRNA library preparation kit for next-generation sequencing as “Illumina Qualified.” “Illumina Qualified” indicates that Illumina’s analysis of libraries prepared with this epMotion method has shown the libraries to perform comparably to those prepared manually. For next generation sequencing systems a variety of sample preparation kits are available. These kits are needed to convert either DNA or RNA samples into sequencing ready libraries, a procedure that includes many steps and can be time consuming. RNA sequencing requires additional steps – either the depletion of unwanted ribosomal or the positive selection of mRNA from total RNA samples. Due to the complexity of the library construction methods, automation is regarded as highly useful.
The epMotion method can be used for the automated construction of 8, 16 or 24 libraries starting with 100 – 1000 ng of total RNA together with Illumina’s TruSeq Stranded mRNA kit. The overall hands-on time is less than 1.5 hours, while the total run time of the entire procedure is ~11.5 hours for 24 samples. Minimized hands-on time & high reproducibility with this automated method help to streamline and standardize the sequencing workflow.
Additional automated methods for NGS library preparation from Illumina are currently in development.